David J. Grdina, Ph.D., M.B.A.
Basic Research
dgrdina at uchicago.edu
Journal Articles
Murley, J.S., D. Nantajit, K.L.
Baker, and D.J. Grdina. Maintenance of Manganese Superoxide
Dismutase (Sod2)-Mediated Delayed Radioprotection Induced by Repeated
Administration of the Free Thiol Form of Amifostine. Radiat. Res., 169:495-505,
2008.
Kataoka, Y., J.S. Murley, K.
Baker, and D.J. Grdina, Relationship Between Phosphorylated
Histone H2AX Formation and Cell Survival in Human Microvascular Endothelial
Cells (HMEC) as a Function of Ionizing Radiation Exposure in the Presence or
Absence of Thiol Containing Drugs. Radiat. Res., 168:106-114, 2007.
Murley, J.S., Y. Kataoka, K.L.
Baker, A. Diamond, W.F. Morgan, and D.J.
Grdina. Manganese
Superoxide Dismutase (Sod2) Mediated Delayed Radioprotection Induced by the
Free Thiol Form of Amifostine and Tumor Necrosis Factor-α. Radiat. Res.,
167:465-474, 2007.
Kataoka, Y., V.P. Bindokas, R.C.
Duggan, J.S. Murley, and D.J. Grdina. Flow Cytometric Analysis
of Phosphorylated Histone H2AX Following Exposure to Ionizing Radiation in
Human Microvascular Endothelial Cells. J. Radiat. Res., 47(3-4): 245-257, 2006.
Little, J.B., D.J. Grdina.
Ionizing Radiation, Chap. 16, in Cancer 7 Medicine, B.C. Decker Inc., Hamilton,
Ontario, CA, Holland-Frei Eds., pp 270-282, 2006.
Murley, J.S., Y. Kataoka, C.J.
Weydert, L.W. Oberley, and D.J. Grdina. Delayed
Radioprotection by Nuclear Transcription Factor kB-Mediated Induction of
Manganese Superoxide Dismutase in Human Microvascular Endothelial Cells After
Exposure to Free Radical Scavenger WR1065. Free Radical Biol. Med.,
40:1004-1016 2006.
Grdina D.J., J.S. Murley, Y. Kataoka, D. Zhou, T.M.
Seed. Radioprotectors: Current Status and New Directions. Radiation Research,
163(6):704-705, 2005.
Murley, J.S., Y. Kataoka, D. Cao,
J.J. Li, L.W. Oberley, and D.J. Grdina. Delayed
Radioprotection by NFkB Mediated Induction of Sod2 (MnSOD) in SA-NH Tumor Cells
after Exposure to Clinically Used Thiol-Containing Drugs. Radiation Research,
162 (5) :536-546, 2004.
Khodarev, N., Y. Kataoka, J.S.
Murley, R.R. Weichselbaum, and D.J. Grdina, Interaction of
Amifostine and Ionizing Radiation on Transcriptional Patterns of Apoptotic
Genes Expressed in Human Microvascular Endothelial Cells (HMEC). Int. J.
Radiation Oncology Biol. Phys., 60(2):553-563, 2004.
Elas M., A. Parasca, D.J.
Grdina, and H.J. Halpern. Oral Administration Is As Effective As
Intraperitoneal Administration of Amifostine in Decreasing Nitroxide EPR Signal
Decay In Vivo. Biochimica et Biophysica Acta, 1637(#2):151-155, 2003.
Grdina D.J, Y. Kataoka, J.S. Murley, K. Swedberg,
J.Y. Lee, N. Hunter, R.R. Weichselbaum, and L. Milas. Anti-Metastatic
Effectiveness of Amifostine Therapy Following Surgical Removal of SA-NH Tumors
in Mice. Seminars in Oncology, 29#6 (Suppl. 18): 2002.
Murley J.S., Y. Kataoka, C.J.
Weydert, L.W. Oberley, and D.J. Grdina. Delayed Cytoprotection
Following Enhancement of MnSOD Gene Expression in SA-NH Mouse Sarcoma Cells
Exposed to Amifostine's Active Metabolite WR1065. Radiation
Research,158:101-109, 2002.
Kataoka, Y., J.S. Murley, N.N.
Khodarev, R.R. Weichselbaum, and D.J. Grdina. Activation of
the Nuclear Transcription Factor kB (NFkB) and Differential Gene Expression in
U87 Glioma Cells Following Exposure to the Cytoprotector Amifostine.
International Journal of Radiation Oncology, Biology, and Physics, 53:180-189,
2002.
Grdina, D.J., J.S. Murley, Y. Katoaka, and D.P.
Calvin, Differential Activation of Nuclear Transcription Factor kB, Gene
Expression, and Proteins by Amifostine's Free Thiol in Human Microvascular
Endothelial and Glioma Cells. Seminars in Radiation Oncology, 12 (No 1, Suppl.
1): 103-111, 2002.
Grdina, D.J., Y. Kataoka, J.S. Murley, N. Hunter,
R.R. Weichselbaum, and L. Milas. Inhibition of Spontaneous Metastases Formation
by Amifostine. International Journal of Cancer, 97:135-141, 2002.
Murley, J.S., Y. Kataoka, D.E.
Hallahan, J.C. Roberts, and D.J. Grdina. Activation of NFkB
and MnSOD Gene Expression by Free Radical Scavengers in Human Microvascular
Endothelial Cells, Free Radical Biology and Medicine,30(No12):1426-1439, 2001.
Mansur, D.B., Y. Kataoka, D.J.
Grdina, and A.M. Diamond. Radiosensitivity of Mammalian Cell Lines
Engineered to Over-Express Cytosolic Glutathione Peroxidase. Radiation
Research, 155(No 4):536-542, 2001.
Grdina, D.J., J.S. Murley, Y. Kataoka, and W.
Epperly. Relationships between cytoprotection and mutation prevention by
WR-1065. Military Medicine, 167(2 Suppl)51-53, 2002.
Grdina, D.J., Y. Kataoka, and J.S. Murley.
Amifostine: Mechanisms of Action Underlying Cytoprotection and Chemoprevention.
Drug Metabolism and Drug Interactions, 16 (No 4): 237-279, 2000
Snyder, R.D. and D.J.
Grdina. Further evidence that the radioprotective aminothiol WR-1065
catalytically inactivates mammalian topoisomerase II. Cancer Research,
60:1186-1188, 2000.
Kataoka, Y., J.S. Murley, R.
Patel, and D.J. Grdina. Cytoprotection by WR-1065, the active
form of amifostine, is independent of p53 status in human malignant glioma cell
lines. International Journal of Radiation Biology, 76:633-639, 2000
Snyder, R.D. and D.J.
Grdina. Further evidence that the radioprotective aminothiol WR-1065
catalytically inactivates mammalian topoisomerase II, Cancer Res.,
60:1186-1188, 2000.
Grdina, D.J., J.S. Murley, Y. Kataoka, and W. Epperly.
Relationships between cytoprotection and mutation prevention by WR-1065.
Military Med., in press, 2000.
Kataoka, Y., J.S. Murley, R.
Patel, and D.J. Grdina. Cytoprotection by WR-1065, the active
form of amifostine, is independent of p53 status in human malignant glioma cell
lines, Int. J. Radiat. Biol.,76:633-639, 2000.
Grdina, D.J., N. Hunter, Y. Kataoka, J. Murley,
and L. Milas. Chemopreventive Doses of Amifostine Confer No Cytoprotection to
Tumor Nodules Growing in the Lungs of Mice Treated with Cyclophosphamide.
Seminars in Oncology, 26 (#2 Suppl. F): 22-27, 1999.
Cai, Y., M. Wu, S.M. Ludeman, D.J.
Grdina, and E. Dolan. Role of O6-Alkylguanine-DNA Alkyltransferase in
Protecting against Cyclophosphamide-induced Toxicity and Mutagenicity Cancer
Res., 59:3059-3063, 1999.
Kataoka, Y., A.M. Diamond, R.R.
Weichselbaum, D.J. Grdina, and D.B. Mansur. Modification of
Anti-Oxidant Enzymatic Activity by Low Dose Selenium Supplementation Does Not
Affect Radiosensitivity of Human Malignant Glioma Cells. Libri Oncologici,
27:63-67, 1998.
Murley, J.S., A. Constantinou,
N.S. Kamath, and D.J. Grdina. WR-1065, an Active Metabolite of
the Cytoprotector Amifostine, Affects Phosphorylation of Topoisomerase IIa
Leading to Changes in Enzyme Activity and Cell cycle progression in CHO AA8
Cells. Cell Proliferation,30:283-294, 1997. 112. Grdina, D.J., J.S. Murley, and
J.C. Roberts. Effect of Thiols on Topoisomerase-IIa Activity and Cell Cycle
Proliferation. Cell. Proliferation, 31: 217-229, 1998.
Liu, S.C., J.S. Murley, G.
Woloschak, and D.J. Grdina. Repression of c-myc Gene
Expression by the Thiol and Disulfide Forms of the Cytoprotector Amifostine.
Carcinogenesis, 18:2457-2459,1997.
Sidjanin, D., D.J. Grdina,
and G.E. Woloschak. UV-Induced Changes in Cell Cycle and Gene Expression within
Rabbit Lens Epithelial Cells. Photochemistry and Photobiology, 63:79-85, 1996.
Halpern, H.J., C. Yu, M. Peric,
E.D. Barth, G.S. Karczamar, J.N. River, D.J. Grdina, and B.A.
Teicher. Measurement of Differences in pO2 Response to Perfluorocarbon/
Carbogen in FSa and NFSa Murine Fibrosarcomas with Low Frequency Electron
Paramagnetic Oxymetry. Radiation Research, 145:610-618, 1996.
Kataoka, Y., J. Perrin, N.
Hunter, L. Milas and D.J. Grdina. WR-2721 Protects Against
Cytoxan-Induced HPRT Mutagenesis Without Affecting Therapeutic Effectiveness.
Seminars in Oncology, 23: Suppl. 8, 53-57, 1996. Diamond, A.M., J.L. Murray, P.
Dale, R. Tritz, Sandstrom, P.A., and D.J. Grdina. The Effects of Selenium on
Glutathione Peroxidase Activity and Radioprotection in Mammalian Cells. Radiat.
Onco. Investigations, 3:383-386, 1996.
Diamond, A.M., J.L. Murray, P.
Dale, R. Tritz, P.A. Sandstrom, and D.J. Grdina. The effects
of Selenium on Glutathione Peroxidase Activity and Radioprotection in Mammalian
Cells. Radiat. Onco. Investigations, 3: 383-386, 1996.
Diamond, A.M., P. Dale, J.L.
Murray, and D.J. Grdina. The inhibition of Radiation-Induced
Mutagenesis by the Combined Effects of Selenium and the Aminothiol WR-1065.
Mutation Research, 356:147-154, 1996.
Schwartz, J.L., J. Cowan, D.J.
Grdina, and R.R. Weichselbaum. Attenuation of G2 Cell Cycle Checkpoint
Control is Associated with Increased Frequencies of Unrejoined Chromosome
Breaks in Human Tumor Cell. Radiation Research, 146:139-143,1996.
Woloschak, G.E., T. Paunesku,
C.R. Libertin, C-M. Chang-Liu, M. Churchill, J. Panozzo, D.J. Grdina,
M.A. Gemmell, and C. Giometti. Regulation of Thymus PCNA Expression is Altered
in Radiation-Sensitive Wasted Mice. Carcinogenesis, 17:2357-2365, 1996.
Grdina, D.J., N. Shigematsu, P. Dale, G.L. Newton,
J.A. Aguilera, and R.C. Fahey. Thiol and Disulfide Metabolites of the Radiation
Protector and Potential Chemopreventive Agent WR-2721 are Linked to Both its
Anticytotoxic and Antimutagenic Mechanisms of Action, Carcinogenesis, 16:
767-774, 1995.
Woloschak, G.E., T. Paunesku,
C.R. Libertin, C.M. Chang-Liu, and D.J. Grdina. Expression of
Thymidine Kinase RNA and a Related Transcript is Modulated by Radioprotector
WR-1065. Cancer Research, 55:4788-4792, 1995.
Murley, J.S. and D.J.
Grdina. Effects of Cycloheximide and WR-1065 on Repair Processes
Related to Cell Survival and Mutagenesis Following Single and Split-doses of
Radiation Exposure. Carcinogenesis, 16:2699-2705, 1995.
Halpern, H.J., G.V.R.
Chandramouli, C. Yu, M. Peric, E.D. Barth, B.A. Teicher, and D.J.
Grdina. Pharmacological compartment viscosity and polarity measured
with very low frequency EPR in tumors of living animals. Magnetic Resonance in
Chemistry, 33:S147-S153, 1995.
Book Chapters
Murley, J.S. and D.J.
Grdina. Chemoprevention with WR-2721 and its Metabolites. In:
Radioprotectors, CRC Press, E. Bump and K. Malaker eds., New York, New York,
pp. 299-313, 1998.
Basic, I., D.J. Grdina,
and T. Lyons. Application of an In Vivo Mutagenesis System to Assess Aminothiol
Effects on Neutron-induced Genotoxic Damage in Mouse Splenocytes. In
Anticarcinogenesis and Radiation Protection: Strategies in Protection from
Radiation and Cancer, O. Nygaard, ed. Plenum Publishing Corporation, New York,
New York, pp. 297-301, 1991.
Nagy, B., D.J. Grdina,
and C.R. Ashman. JANUS Neutron Irradiation of a Mouse Cell Line Containing a
Shuttle Vector Plasmid. In Anticarcinogenesis and Radiation Protection:
Strategies in Protection from Radiation and Cancer, O. Nygaard, ed., pp. 85-92,
Plenum Publishing Corporation, New York, 1991.
Grdina, D.J., B. Nagy, and P.J. Meechan. Effect of an
Aminothiol (WR1065) on Radiation-Induced Mutagenesis and Cytotoxicity in Two
Repair-deficient Mammalian Cell Lines. In Anticarcinogenesis and Radiation
Protection: Strategies in Protection from Radiation and Cancer, O. Nygaard,
ed., pp. 287-295, Plenum Publishing Corp. New York, 1991.
